Medico Guides 8th Block (Module No 17) Microbiology Guidelines

by

Prepared by:

                         Kisa e Zahra (G12)

Compiled by:

                   Hafiz Muhammad Umair Noor (G12)

Reference Books:

  • Anwar Microbiology 2nd Edition
  • Sketchy Microbiology

Note:

  • Sketchy + Anwar (this is the best combo for micro and you have to prepared everything in this) 
  • You can also use Levinson as it is standard book for your better understanding.

(ID-Pa-001)

For clinical bacteriology basic steps are always on your fingertips like staining, morphology, virulence factors, special disease by microbe etc

Gram +ve Microbes

Anwar Chapter # 12, 14

Levinson Chapter # 15, 17 (All tables are imp)

IMPORTANT POINTS FOR BACTERIA

  • Staph aureus ( all diseases caused by this(Kawasaki disease and other), its cell wall components and pathogenisis
  • For strep know about it’s hemolysis classification and strep pyogenese is imp one
  • Clostridium classification+imp diseases caused by them
  • Bacillus (imp) caused by reheated rice
  • Corynebacterium pathogenisis and diseases imp
  • Read of listeria and gardenella

Gram -ve Cocci

Anwar  Chapter # 13

Levinson Chapter # 16

  • Neisseria species are imp (specially their pathogenesis and lab diagnosis)

Gram -ve Rods:

Lab diagnosis are important that will differ microbes like MacCkoney’s agar, urease test, TSI test colour.

Anwar Chapter #15

Levinson Chapter # 18+19

  • E.coli (most imp) it’s pathogenesis, it’s ETEC and EHEC form and lab diagnosis
  • Salmonella (imp pathogenesis, rose spots, lab diagnosis)
  • Shigella (pathogenesis)
  • Vibrio (imp pathogenesis+ rice watery stools + lab diagnosis)
  • Proteus (pathogenesis+ urease positive + staghorn canaliculi)
  • Pseudomonas (imp pigments + diseases + patho)
  • H.pylori and camplylobacter (read+ sketchy)
  • Actinomyces (only sketchy)

Spirochetes:

Anwar Chapter # 21

Levinson Chapter # 24

  • Traponema (imp one it’s types, patho, lab diagnosis)
  • Borrelia and leptospira (read)

Mycobacterium:

Anwar Chapter # 18

Levinson Chapter # 21

  • Mycobacterium Tuberculosis is most imp . Should done everything ( pathogenesis, diseases, lab diagnosis imp)
  • Laprae( imp and it’s two types difference)

Rickettsia and Chlymadia:

Anwar Chapter # 22, 23

  • Just do sketchy and anwar

(D-Pa-002)

  • For parasites most imp are life cycles and the most of transmission
  • Sketchy and Anwar are best for context and do life cycles from Levinson

Anwar Chapter # 44, 45, 46, 47, 48

Levinson Chapter # 51, 52, 54, 55, 56

IMPORTANT PARASITES

  • Entamoeba
  • Plasmodium (very imp)
  • Echinococcus(life cycle imp)
  • Shictosoma
  • Entrobius
  • Ascaris
  • Necator
  • Ancylostoma

(Note:  do each and everything of above mention parasites)

(ID-Pa-003)

For fungi (Anwar + sketchy is enough)

Anwar Chapter # 40, 41, 42

IMPORTANT FUNGI

  • Dermatophytes (type+ lab diagnosis)
  • Histoplasma imp (morphology+ pathogenesis)
  • Candida (most imp) (patho+ clinical findings+ lab diagnosis)
  • Cryptococcus (patho+ lab diagnosis)
  • Aspergillus (imp patho+ it’s forms + lab diagnosis)

(ID-Pa-004)

  • Virology is tough from others microbes just do imp viruses
  • Sketchy and Anwar is more than enough
  • Always remember imp points that will help in diagnosis

Anwar Chapter # 31

  • Herpes+ varicella + cytomegalovirus are imp one
  • Others just read

Anwar Chapter # 32

  • Influenza (important it’s types+ antigenic drift and shift and pathogenisis factors
  • Measles mumps (sketchy is enough and read from Anwar)
  • Rabies most imp) (it’s pathogenesis and incubation period and vaccine)
  • Rubella (read)

Anwar Chapter # 33

  • Corona virus (important it’s pathogenesis)

Anwar Chapter # 34

  • Polio virus (imp) (it’s mode of transmission + pathogenesis + it’s vaccine types)
  • Reovirus (read)

Anwar Chapter # 35

  • Hepatitis B is most imp among them
  • Serological findings
  • Clinical features
  • Pathogenesis
  • Window period
  • Read other hepatitis types

Anwar Chapter # 36

  • Dengue virus (very imp)
  • Dengue hemorrhagic fever hypothesis
  • Lab diagnosis

Anwar Chapter # 37

  • Just do table

Anwar Chapter # 38

  • HIV (most imp)
  • Pathogenesis
  • Cycle
  • Stages of AIDS
  • Lab diagnosis

(Note: Do sktechy must for microbiology)

(ID-Pa-005)

Microbes Causing CNS Infections

For meningitis this will help:

  1. Less than 2 Months (Neonates and Young Infants)
  1. Streptococcus Agalactiae (Group B Streptococcus
  2. Escherichia Coli (E. coli)
  3. Listeria Monocytogenes
  4. More than 2 Months (Infants and Young Children)
  1. Streptococcus Pneumoniae (Pneumococcus)
  2. Neisseria Meningitidis (Meningococcus)
    • Important Note: If a child is unvaccinated then Haemophilus influenzae becomes a very significant, often the number 1 cause of bacterial meningitis in this age group.
  3. 2-18 Years (Children and Adolescents)
  4. Neisseria Meningitidis (Meningococcus)
  5. More than 18 Years (Adults)
  6. Streptococcus Pneumoniae (Pneumococcus
  7. Immunocompromised Individuals (e.g. HIV positive, Drug Users)
  8. Listeria Monocytogenes

(Note: Correlate above knowledge)

(ID-Pa-006)

  • GIT problems
  • Correlate above knowledge
  • Hepatitis guidelines mention in virology part
  • Entamoeba in parasitic part

(ID-Pa-007)

  • GIT infections
  • Correlate from above knowledge

(ID-Pa-008)

  • Rickettsia and leptospira mention in above bacteriology guidelines
  • Anthrax, plague,francisella, bartonella and Brucella (do table from Anwar chapter17 and sketchy)
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