Prepared by:
Hanzala Masood (G14)
Compiled by:
Muhammad Ahmad Bhatti (G14)
Reference Books:
- Lippincott Illustrated Reviews: Biochemistry 8th Edition (Chapter numbers are mentioned according to this edition)
- Satyanarayana Biochemistry 6th Edition
- Harper’s Illustrated Biochemistry 30th Edition
- Mark’s Biochemistry 6th Edition
- Lehninger Principles of Biochemistry 7th Edition
- Lippincott Illustrated Reviews: Cell and Molecular Biology 2nd Edition
ENDOCRINE BIOCHEMISTRY
NOTE: You can also cover biochemistry portion of Endocrinology from Medicnetic or Medico Express if you feel that material according to these guidelines are scattered. I covered this portion entirely from these guidelines and PDFs so I can’t say anything about Medico Express or medicnetic. Only go for these books if content is good enough.
(EnR-B-001)
- Do this from pics/pdf shared
(EnR-B-002)
- Do this from Satya Chapter 19
- You can also do this from Harper chapter 42 (pics are shared)
(EnR-B-003a)
Signalling pathways:
- Do this from pdf/pics shared
(EnR-B-003b)
Chapter 23: (Insulin & Glucagon)
- Do this from Lippincott chapter 23
- Insulin synthesis v.v.v.imp
- Can also do from Guyton chapter 79
(EnR-B-003c)
Chapter 21: (Epinephrine)
- Do this from Lippincott chapter 21(III-A) page no 317 to 319
(EnR-B-003d & 006)
(Growth, Thyroid & Steroid Hormones)
- Do these from Guyton chapter 76, 77 & 78
- You can also do cortisol and other steroid hormones from Lippincott chapter 18 (VII) pg no 262 to 266
(EnR-B-004)
- This is a general LO. It will be covered by doing other LOs.
(EnR-B-005)
Chapter 78: (Guyton)
- Do this from Guyton chapter 78
- Pro-opiomelanocortin gene topic complete
(EnR-B-007)
- Do these from pics/pdf shared
GENETICS
(EnR-B-008 to 014)
- Do these from the pics/pdfs shared
(EnR-B-015 to 016)
- The topic of Karyotypes is not given in the book. Do it from pics shared.
- As far as mutations are concerned, some of them are given in Lippincott chapter 32 (Pg 498 & 499). Rest will be shared as pics.
(EnR-B-017 to 021)
Chapter 30: (Lippincott)
- No need to do the the structure of DNA as it is not the part of ur syllabus
- Start from (III-Steps in ….) pg no 462 to 475
- Although whole chapter is imp but the most important topics are below:
- Replication strand and required proteins ( DNA A , DNA helicase and Single strand binding protein) v.imp
- Topoisomerases type 1 and 2 (their difference also and their inhibitor green box) and supercoilimg (v.v.imp)
- Leading and Lagging strand
- Primase and primosome
- DNA Polymerase III ( proofreading and exonuclease activity) imp
- DNA ligase
- Eukaryotic DNA replication complete
- Telomeres and Telomerases v.v.imp (cover entire topic given in eukaryotic DNA replication)
- Eukaryotic DNA organization
- DNA Repair (v.v.imp)
Many mcqs are given from this topic
- Cover complete topic all 4 types and their clinical correlates i.e Xeroderma Pigmentosum & Lynch Syndrome (v.v.imp)
- Exonuclease & Endonuclease imp
- For Central Dogma Concept You can use medicnetic/ medico express
- Fig 30.21, 30.23, 30.24 (v.v.imp)
(EnR-B-022 to 024)
Chapter 31: (Lippincott)
- No need to do structure of RNA as not a part of syllabus
- Start chapter from (III-Prokaryotic) pg no 484 to 493
- Prokaryotic Gene Transcription
- Prokaryotic RNA polymerase
- RNA synthesis (Complete)
- Associated antibiotics (Rifampin and Dactinomycin) v.imp
- Eukaryotic Gene Transcription
- Eukaryotic RNA polymerases 1,2,3
- Promoters, Transcription factors, Enhancers and Inhibitors associated with RNA polymerase II
- Post Transcriptional Modification of RNA (v.v.imp)
(EnR-B-025 to 027)
Chapter 32: (Lippincott)
- Skip initail headings of Genetic code
- Start chapter from Mutations
- Cover All mutations and their examples given in the text (v.imp)
- Skip components required for translation and start from Codon Recognition by Transfer RNA
- Wobble Hypothesis (v.v.imp) for both written and viva
- Steps in translation including initiation (it’s sequences also), elongation and termination
- Shine Dalgarno sequence (imp)
- For Steps of Translation Fig 32.13 is quite helpful
- Steps of fig 32.13 and Actions Of 5 Antibiotics given in fig 32.13 (v.imp)
- Co and Posttranslational modifications ( v.imp)
(EnR-B-028)
Chapter 33: (Lippincott)
Understand Regulatory sequences and molecules first
- Regulation Of Prokaryotic Gene Expression (imp)
- Operon definition
- Operator and Inducer
- Lactose Operon (Lac Operon) it is v.v.v..imp topic (Cover this topic completely including it’s regulation imp) and Fig 33.4 v.v.imp
- Tryptophan Operon (v imp) complete
- Regulation Of Eukaryotic Gene Expression (imp)
Although only 2-3 topics are important from this portion but in LOs it is not clearly mentioned that only that 2-3 topics are included so it is preferable to do this completely at least once with special emphasis on following topics
- Messenger RNA editing ( v.imp)
- Messenger RNA stability
- Iron Metabolism (v.v.v.imp & our Proff Seqs) shortlist on fig 33.14 as it is enough
- RNA interference & therapeutics (v.v.imp) shortlist on fig 33.15 v.imp
(EnR-B-029)
Chapter 34: (Lippincott)
- Don’t do complete chapter as not in syllabus
- Do DNA Cloning pg 534 to 537 (Fig 34.6)
- Do DNA Sequencing from pg 539 (Fig 34.9 only)
- Do Southern Blotting complete topic on pg 540 ( v.v.imp)
- It will also cover Northern & Western Blotting. Also do Fig 34.25 imp
- Do RFLP from pg 541 to 547
- Do PCR from pg 547 to 550 v.v.v.imp topic (Important for both written and viva and don’t skip even a single thing from this topic)
- Do Human Genome Project from Satya chapter 28